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Sample preparation of mango leaf extract

Accurately weigh 400g Mangifera Indica Extract, add 8 times the amount of 70% ethanol solvent, soak for 1 hour, boil and reflux for 2 hours, filter while hot, add 8 times the amount of 70% ethanol solvent to the filter residue, continue to reflux for 2 hours, combine the filtrate for 2 times, put it at room temperature, accurately measure the volume of the extract solution to a total of 6.86l, and concentrate to 800ml according to the ratio of crude drug (Gardenia Yellow): solution volume (ML) = 1:2 , 3000 R / min, centrifugation for 15 min to discard the precipitate. Accurately measure 800ml of D101 macroporous adsorption resin pretreated, and wet column.

Take the sample of the treated concentrated solution, the Astaxanthin Supplier is 40min / BV, pre elute with 3bv water, elute with 5 times of 15% ethanol, recover ethanol from 15% ethanol eluent and concentrate to the end, weigh 10g of mangiferin crude product, heat and dissolve with 20ml of 50% ethanol solution to make the solution supersaturated, stand, cool and filter, scrape off the filter cake, wash the sediment with 20ml of 50% ethanol solution, mix well Evenly, filter again, put the filter cake into the vacuum drying oven for drying at 60 ℃, and obtain the Mango Leaf Extract sample.

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Physiological function of ferulic acid(3)

Antibacterial and antiviral functions

Hirabayashi et al. Found that the experimental mice were infected with influenza virus, and the control group was treated with Ferulic Acid and isoferulic acid. Analysis of the results showed that the production of interferon in the experimental group decreased rapidly. In recent years, there are many reports that ferulic acid has a significant inhibitory effect on cold virus (IV), respiratory syncytial virus (Spirulina Powder) and HIV. In the same cell line, we studied the relationship between ferulic acid and inflammatory protein. Results Flax lignans can dramatically reduce the production of egg white. Among them, ferulic acid has an inhibitory effect on HIV, which makes it possible for N-Acetylneuraminic Acid to become a chemical therapeutic agent in the future. It is speculated that the mechanism of ferulic acid’s inhibition on the virus is related to its ability to reduce the activity of xanthine oxidase, which is due to the fact that ferulic acid can generally lead to the occurrence of some inflammation. It is speculated that ferulic acid’s antibacterial function is mainly due to its strong inhibition on n-ethylhexyltransferase in bacteria.

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Physiological function of ferulic acid(2)

Antioxidant function

Metabolism is the characteristic of living body. At the same time, living body is always attacked by a Ferulic Acidctive oxygen substances (molecules or free radicals directly or indirectly transformed from molecular oxygen, which are more active than molecular oxygen). Free radicals (also known as free radicals, which refer to the Capsicum Oleoresin, atomic groups or molecules in special states with unpaired electrons in the outer orbit), Both of these two substances can directly participate in the formation of tumor or induce the production of carcinogens, change the DNA of life body, activate the proto oncogene, so as to promote the body to produce cancer cells and reduce the Sweet Potato Powder is the key link of prevention and treatment of related diseases. Some studies have shown that ferulic acid can kill free radicals skillfully and restore the normal functions of living bodies. Ferulic acid can inhibit the enzymes that produce free radicals in living bodies. On this basis, it can also increase the enzymes that clear free radicals. At the same time, Mixed Tocopherols Oil can greatly enhance the activities of waking reductase and glutathione transferase, and control the proportion of active tyrosinase. Zhou et al. Showed that ferulic acid had significant antioxidant effect and had good scavenging effect on ammonia peroxide, superoxide radical, hydroxyl radical and nitroperoxide.

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Physiological function of ferulic acid(1)

Anti radiation effect

Radiation-induced organ failure is largely caused by chronic oxidative damage. Radiation damage to the body can be divided into direct damage and indirect damage. The direct damage is that radiation directly causes the rupture of some sensitive molecules in the cell; the indirect damage is that Capsicum Oleoresin causes the radiolysis of water, which leads to the rise of intracellular reactive oxygen species and then causes the change of subcellular structure. Therefore, antioxidants are widely used in the treatment of radiation damage. To protect cells from ROS damage, it is necessary to maintain the homeostasis of endogenous thiol pool, especially the content of glutathione (GSH) and nicotinamide adenine dinucleotide phosphate (Capsicum Oleoresin). Glutathione provides reduction equivalent for the conversion of hydrogen peroxide and lipid peroxide into water and lipid alcohol, and protects thiol protein group from oxidative damage. The rate limiting reaction of glutathione biosynthesis is catalyzed by glutamate cysteine ligase (Sweet Potato Powder), which consists of a catalytic subunit (GCLC) and a regulatory subunit (GCLM). Nicotinamide adenine dinucleotide phosphate is an important antioxidant in tissues, which can maintain the redox potential of cells by reducing the reduction equivalent of glutathione reductase and thioredoxin.

Ferulic Acid, as a phenolic plant component, has strong antioxidant activity, and has a great role in promoting human health. Ferulic acid can significantly increase the content of glutathione and nicotinamide adenine dinucleotide phosphate in irradiated cells, which has a protective effect on irradiated endothelial cells. Heme oxygenase is an antioxidant enzyme, which can convert heme into biliverdin, and finally into antioxidant bilirubin. Ferulic acid can regulate the expression of this enzyme, so as to play a protective role in radiation protection.

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Introduction to the functions of citrus aurantium extract(2)

Mild stimulant Citrus Aurantium Extract

Studies have shown that the enhanced energy effect of simephrine can stimulate the central nervous system. This integration may include improving blood circulation through the heart and Sweet Potato Powder, raising blood pressure and improving mental activity, which make simephrine easy to use as a mild stimulant.

Digestive system

According to the traditional method of use, Fructus aurantii extract can stimulate gastric function by relaxing and reducing flatulence, so as to improve gastrointestinal system activity. Sweet Potato Powder may also help reduce nausea and eliminate stomach turbulence such as flatulence and bloating.

Sterilization function

Fructus aurantii Mixed Tocopherols Oil is a nontoxic organic sterilization product. It can inhibit the growth of bacteria and the infection of some viruses. Therefore, the extract is widely used as a disinfectant, antiseptic for food or cosmetics, fungicide, antibacterial agent, fungicide and anti filter agent.

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Introduction to the functions of citrus aurantium extract(1)

Synephrine is the main active component in the fruit of lime. Citrus Aurantium Extract can effectively prevent excess energy (heat accumulation), regulate qi by wind, warm stomach, promote appetite and accelerate metabolism. In theory, lime can accelerate fat metabolism and will not have adverse side effects on cardiovascular system like the patients using ephedra. White Kidney Bean Extract is also a mild fragrant expectorant, a neurotranquilizer and a laxative for constipation.

Lose weight

The most likely explanation for the weight loss function of lime extract is the amphetamine like effect of alkaloids. Although this effect is likely to be slightly less active than ephedrine (Flax lignans), and less active than ephedrine, users can expect variable effects including consumption of excess calories, decreased appetite and increased satiety, all of which may lead to weight loss. A study of dogs has also shown that synephrine can increase metabolic rate in special fatty tissue called brown fat tissue.

Due to the fact that synephrine and other components in trifoliate are structurally similar to ephedrine and act as special adrenaline receptor agonists (N-Acetylneuraminic Acid, but not Beta-1, Beta-2 or Alpha-1), trifoliate does not have the same negative effect on the central nervous system as ephedra. Ephedra will stimulate all the beta adrenoceptors.

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The effect of bitter melon extract

Balsam pear element was originally made by processing balsam pear extract, but the effect and effect of Gardenia Yellow pear element are superior to balsam pear, because it is more simple to be absorbed by human body after concentration(Mangiferin); besides the effect of clearing away heat, detoxification and eyesight, there are many pharmacological effects;

Bitter Melon Extract element was originally made by processing balsam pear extract, but the effect and effect of balsam pear element are superior to balsam pear, because Mixed Tocopherols Oil is more simple to be absorbed by human body after concentration; besides the effect of clearing away heat, detoxification and eyesight, there are many pharmacological effects;

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Preparation of mango leaf extract toothpaste

At present, the new health care products or drugs developed with Mango Leaf Extract as the main component are generally oral preparations. Because the bioavailability of mangiferin is very low, the clinical trial effect of these preparations is not ideal.

A. Glue making: according to the amount in the Gardenia Yellow, put glycerin, 70% sorbitol solution, xanthan gum and sodium carboxymethylcellulose into the glue making pot, stir at high speed for 7 minutes, then add water, xylitol, sodium pyrophosphate, potassium sorbate and citric acid, continue to stir until Ginkgo Biloba Extract is colloidal, stand for 6 hours, and then stir at high speed for 20 minutes to get transparent and uniform glue.

B. Kneading: pour the glue into the kneader, add other components in the formula, mix evenly, and get the sticky paste.

C. Grinding: put the paste into the colloid mill and grind until Red Beet Root Powder becomes a uniform and soft paste.

D. Aging: transfer the ground paste into a temporary storage tank, and allow it to stand for 12 hours, so that the bubbles in the paste will be discharged, and the paste will become more viscous and uniform.

E. Vacuum degassing: pour the aged paste into the vacuum degasser, and turn on the machine to remove the bubbles in the paste.

F. Filling: put the vacuum degassed paste into the automatic filling machine, separately put it into the toothpaste tube, and then seal the tail to get the product mango leaf extract toothpaste.

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Detection and analysis method of ferulic acid(2)

3. Thin layer spectrophotometer method

The qualitative analysis of Ferulic Acid extracted from rye bran was carried out by tlc-spectrophotometry. The developing agent was dichloromethane: acetonitrile: formic acid = 75:25:10. The quantitative analysis was carried out by Flax lignans. The results showed that although the spectrophotometer was easily interfered by other components, the relative error of Capsicum Oleoresin was about 7%, and the reproducibility was good 。

4. High performance capillary electrophoresis

Capillary zone electrophoresis is the most widely used separation mode. Features simple, efficient, fast, less sample consumption, Sweet Potato Powder. The content of ferulic acid in angelica preparation was determined by hollow fused quartz capillary tube. The results showed that the content of ferulic acid in angelica preparation could be quantitatively determined in the range of 5-100ug \ ml, with good repeatability.

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Detection and analysis method of ferulic acid(1)

1. High performance liquid chromatography

The content of Ferulic Acid was determined by HPLC. The method is simple, rapid, accurate and precise. It is introduced in the literature that the mobile phase is mostly acid system, mainly including methanol water phosphoric acid system, methanol water glacial acetic acid system, methanol acetonitrile water glacial acetic acid system, etc. the amount of methanol can be adjusted properly in the experiment. The content of ferulic acid in Compound Ginkgo Oral Liquid was determined by HPLC. The Mixed Tocopherols OilFerulic Acid was methanol: 1% glacial acetic acid (45:55). The detection wavelength was 320nm, the flow rate was 1.0ml/min, and the column temperature was 25 ℃. The linear relationship of ferulic acid oxygen intake was good in the range of 0.176-0.88ug.

2. Thin layer scanning

Thin N-Acetylneuraminic Acid is also one of the commonly used methods for the determination of ferulic acid. The method is rapid, but its sensitivity is not ideal. Ji Shengguo et al. Determined the content of ferulic acid in Jiangzhi Tongmai oral liquid by TLC scanning. Using Flax lignans acetic acid chloroform (6:0.5:3.5) as developing agent, the single wavelength reflection hair saw tooth scanning was carried out with the scanning wavelength of 325nm. Good stability.

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