标签： Ferulic Acid

Anti radiation effect

Radiation-induced organ failure is largely caused by chronic oxidative damage. Radiation damage to the body can be divided into direct damage and indirect damage. The direct damage is that radiation directly causes the rupture of some sensitive molecules in the cell; the indirect damage is that Capsicum Oleoresin causes the radiolysis of water, which leads to the rise of intracellular reactive oxygen species and then causes the change of subcellular structure. Therefore, antioxidants are widely used in the treatment of radiation damage. To protect cells from ROS damage, it is necessary to maintain the homeostasis of endogenous thiol pool, especially the content of glutathione (GSH) and nicotinamide adenine dinucleotide phosphate (Capsicum Oleoresin). Glutathione provides reduction equivalent for the conversion of hydrogen peroxide and lipid peroxide into water and lipid alcohol, and protects thiol protein group from oxidative damage. The rate limiting reaction of glutathione biosynthesis is catalyzed by glutamate cysteine ligase (Sweet Potato Powder), which consists of a catalytic subunit (GCLC) and a regulatory subunit (GCLM). Nicotinamide adenine dinucleotide phosphate is an important antioxidant in tissues, which can maintain the redox potential of cells by reducing the reduction equivalent of glutathione reductase and thioredoxin.

Ferulic Acid, as a phenolic plant component, has strong antioxidant activity, and has a great role in promoting human health. Ferulic acid can significantly increase the content of glutathione and nicotinamide adenine dinucleotide phosphate in irradiated cells, which has a protective effect on irradiated endothelial cells. Heme oxygenase is an antioxidant enzyme, which can convert heme into biliverdin, and finally into antioxidant bilirubin. Ferulic acid can regulate the expression of this enzyme, so as to play a protective role in radiation protection.

3. Thin layer spectrophotometer method

The qualitative analysis of Ferulic Acid extracted from rye bran was carried out by tlc-spectrophotometry. The developing agent was dichloromethane: acetonitrile: formic acid = 75:25:10. The quantitative analysis was carried out by Flax lignans. The results showed that although the spectrophotometer was easily interfered by other components, the relative error of Capsicum Oleoresin was about 7%, and the reproducibility was good 。

4. High performance capillary electrophoresis

Capillary zone electrophoresis is the most widely used separation mode. Features simple, efficient, fast, less sample consumption, Sweet Potato Powder. The content of ferulic acid in angelica preparation was determined by hollow fused quartz capillary tube. The results showed that the content of ferulic acid in angelica preparation could be quantitatively determined in the range of 5-100ug \ ml, with good repeatability.

1. High performance liquid chromatography

The content of Ferulic Acid was determined by HPLC. The method is simple, rapid, accurate and precise. It is introduced in the literature that the mobile phase is mostly acid system, mainly including methanol water phosphoric acid system, methanol water glacial acetic acid system, methanol acetonitrile water glacial acetic acid system, etc. the amount of methanol can be adjusted properly in the experiment. The content of ferulic acid in Compound Ginkgo Oral Liquid was determined by HPLC. The Mixed Tocopherols OilFerulic Acid was methanol: 1% glacial acetic acid (45:55). The detection wavelength was 320nm, the flow rate was 1.0ml/min, and the column temperature was 25 ℃. The linear relationship of ferulic acid oxygen intake was good in the range of 0.176-0.88ug.

2. Thin layer scanning

Thin N-Acetylneuraminic Acid is also one of the commonly used methods for the determination of ferulic acid. The method is rapid, but its sensitivity is not ideal. Ji Shengguo et al. Determined the content of ferulic acid in Jiangzhi Tongmai oral liquid by TLC scanning. Using Flax lignans acetic acid chloroform (6:0.5:3.5) as developing agent, the single wavelength reflection hair saw tooth scanning was carried out with the scanning wavelength of 325nm. Good stability.

Sweet Potato Powder has been proved that saffron has anticancer effect. The Spirulina Powder effect of the total extract of saffron ethanol and different components of saffron on the growth of human tumor cells in vitro was studied. The results showed that the half lethal dose of different components to HeLa cells were: Capsicum Oleoresin 2.3mg/ml of saffron dry stigma extract, 3 mmol / L of saffron, 0.8mmol/l of saffron aldehyde and 3 mmol / L of saffron bitter.

Modern research has found that saffron preparation has obvious anti-cancer and anti-cancer ability, and its anti-cancer mechanism may be to destroy the synthetase system, that is, to destroy a protein link medium which is essential for cell synthesis, so as to inhibit the synthesis of and, and achieve the effect of inhibiting the growth of tumor cells.

Ferulic Acid, crocin also inhibited the activity of protein kinase and the expression of proto oncogene. It was found that saffron extract can inhibit the growth of human tumor cells. The results showed that the cells treated with saffron had swelling and plasma membrane rupture, which indicated that the cytotoxicity of the cells might be caused by the absorption of extracellular fluid. Considering water solubility and high inhibition, crocin is considered to be one of the most promising cancer drugs in crocin.

Garcia Olmo et al. Used crocetin and carotenoid glycosides extracted from Crocus drying stigma for a long time to treat rats inoculated with colon cancer. It was found that crocetin and carotenoid glycosides can prolong the life span of female rats and slow down the growth rate of cancer cells, but they have no effect on male rats. Immunoserum analysis showed that the two compounds had only slight toxicity to kidney.